Fatemeh Zeinali Sehrig; Mohammad Zaefizadeh; Changiz Ahmadizadeh; Mohammad Reza Alivand; Saeid Ghorbian
Volume 25, Issue 8 , 2023
Abstract
Background: Breast cancer (BC) is a malignant tumor that occurs in the epithelial tissue of the breast gland and has become the most common malignancy in women. Various studies have reported the effect of epigenetic changes, including DNA methylation and microRNAs, on breast carcinogenesis. microRNAs ...
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Background: Breast cancer (BC) is a malignant tumor that occurs in the epithelial tissue of the breast gland and has become the most common malignancy in women. Various studies have reported the effect of epigenetic changes, including DNA methylation and microRNAs, on breast carcinogenesis. microRNAs play an important role in the post-transcriptional regulation of genes and are important regulators of oncogenic pathways. Studying microRNAs in BC facilitates the development of targeted therapies and early detection of this cancer.
Objectives: This study aimed to evaluate the expression level of miR-508-5p and miR-635 in BC tumor tissues compared to healthy marginal tissues.
Methods: In silico analysis confirmed microarray datasets (GSE40525, GSE44124 and GSE45666) downloaded from the GEO database. The analysis was defined using the Affy packages in R software to screen remarkably dysregulated miRNAs attended by utilized to predict the potential biological processes and molecular pathways of miR-508-5p and miR-635. Experimental statistical significance of differences in miRNA relative expression results was analyzed by pair-wise fixed reallocation randomization test as a statistical model included in the REST (relative expression software tool).
Results: GEO microarray data set, similar to qPCR results, showed that miR-508-5p was downregulated in the sample group by a mean factor of 0.327 (S.E.M range is 0.031-2.000). Moreover, miR-635 was upregulated in the sample group by a mean factor of 2.361 (S.E.M range is 0.250-16.000).
Conclusion: miR-508-5p was downregulated, while miR-635 was upregulated in BC tissues. They may be proposed as diagnostic and therapeutic biomarkers for patients with BC.
Changiz Ahmadizadeh; Hamid Mirzaei
Volume 20, Issue 3 , 2018, Pages 1-7
Abstract
Background: Lactobacilli are commonly used organisms in the production of probiotics. Objectives: The present study was conducted to examine the effect of probiotics isolated from dairy products on the expression of Stx1 and Stx2 genes in verotoxigenic E. coli (VTEC). Methods: This experimental study ...
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Background: Lactobacilli are commonly used organisms in the production of probiotics. Objectives: The present study was conducted to examine the effect of probiotics isolated from dairy products on the expression of Stx1 and Stx2 genes in verotoxigenic E. coli (VTEC). Methods: This experimental study was conducted during April and January 2016 in East Azerbaijan, Iran. At first, Hundred samples of traditional dairy products were collected. Then, using standard phenotypic and genotypic methods, the Lactobacillus casei (L. casei) and lactobacillus (L. plantarum) were studied. Subsequently, these strains were examined along with the pathogenic bacteria strain of Escherichia coli (E. coli) (O157: H7) by neighboring cultivation. After extraction of mRNA, the expression of these genes was determined using real- time PCR.Results: The results revealed that L. casei reduces the expression of Shiga toxin (Stx1 and Stx2) genes more compared to L. plantarum (P = 0.001). Moreover, it was found that both Lactobacilli reduce the expression of Stx1 genes more than Stx2. Conclusions: Probiotics could be used to prevent and control intestinal diseases caused by E. coli
